DNA

Part:BBa_K5310022:Design

Designed by: Elias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-26)


T3 hairpin arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 17
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed by our team based on complementarity with the rest of the Y hairpin, the H1 arm of the HCR hairpin as well as stability standards (thermodynamics, closing base pairs, loop size).


Source

The structure may not be as stable in vivo as it is shown to be in NUPACK and in vitro experiments.

References